Å·ÃÀAV

Introduction

From Sample Prep, HPLC/UHPLC to MS Instrumentation

Part I. Sample Preparation in LC-MS :

• The role/benefits of sample preparation in good LC-MS practice
• Overviews of different sample preparation techniques and their applicability
• Guide to selecting the right Sample Preparation method

Part II. Intro to Liquid Chromatography :

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Survey of different LC bonded phases and their application
• Guide to selecting the right LC column for your application

Part III. Introduction to LC-MS :
Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

• Atmospheric Pressure ​Ionisation theory, techniques and methods:
  • Desolvation
  • Electrospray Ionisation (ESI)
  • Atmospheric Chemical Ionisation (APCI)
•  Mass Analysis
  • The importance of calibration, and the differences between nominal mass, accurate mass and mass resolution
• Mass Analysers
  • Quadrupole – benefits, limitations and general usage
  • Time of Flight – benefits, limitations and general usage
• Data analysis
  • Quantitation
  • Application of targeted techniques eg. MRM to data acquisition and processing
  • Qualitative approaches to identification
  • Application of discovery techniques eg. scanning, information dependent acquisition, library searching

Useful links

Venue

The Principal Hotel, Manchester

The Principal Hotel, Manchester, Oxford St, Manchester, M60 7HA, United Kingdom

Useful links

Organised by

Contact information

Mark Comber
Phenomenex
Melville House,
Queens Avenue
Macclesfield
SK10 2BN
1625504070
Contact us by email

---