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A highly interactive and in depth course which aims to provide the practical skills and knowledge necessary to develop better methods and method development strategies for high performance liquid chromatography
 

Who should take this course?

If you've ever wished that HPLC or UHPLC method development was more logical and methodical, this is the course for you. This course is the ‘gold standard’ in LC method development courses and is for anyone who has some involvement in developing new methods, troubleshooting older methods or transferring existing methods into a routine laboratory. Attendees should have at least some LC experience in the laboratory.
 

What does it cover?

This two day course focuses on the fundamentals of chromatographic separation and applies them in a time-proven strategy, using the principles of QbD to enable faster development of better, more robust HPLC and UHPLC methods. This two day course includes a number of interactive workshops and discussions that encourage a thorough understanding of the topics covered. Delegates will be introduced to LC method development software and shown how it can be used to help them find the optimum separation conditions. During the course, delegates will solve a series of real-world LC method development problems using chromatographic computer modelling, demonstrating how effectively this approach to method development works. The principles used in the computer modelling examples are fully applicable and translatable to the real-world separations and separation strategies in your laboratory.
 

What will I get from this course?

Based on the instructor’s extensive experience in the laboratory supporting pharmaceutical and bioanalytical method development, those attending this course will gain the practical skills needed to develop better HPLC and UHPLC methods in a shorter time. The important principles of resolution will be explored in order to provide a solid platform from which delegates will be able to implement a systematic, efficient approach to developing rugged, robust reversed-phase LC methods. The techniques can be used as a stand-alone method development strategy or added to existing procedures to help streamline method development processes. On this course you will be shown how to achieve the best possible chromatography, save hours of frustration and improve your results time after time.

 Quality by Design?

The central tenet of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC or UHPLC method, QbD strategies can be used to guide the method development process resulting in a standardised procedure, that provides better methods more quickly, that are more easily validated and are easier to use and adjust in routine applications afterwards.
 

Course Outline 

Day 1
Introduction to LC method development
HPLC/UHPLC basics
HPLC/UHPLC columns
Reversed-phase separations for neutral molecules
Reversed-phase separations for ionic samples
Equivalent and orthogonal columns
 
Day 2
 Gradient elution
UHPLC Considerations
Quality by Design
Normal-phase and HILIC
 
Bonus Options for the course:-
Chiral, Ion-exchange and prep
Quality issues
LC-MS & LC-MS/MS
Sample preparation
 
During the course the core sections will always be covered. Then, depending on the interests of the particular group of attendees and the remaining time, one or more of the Bonus topics may be included.
 
The main goal of this course is to provide valuable, practical help to attendees that can be applied in real situations in real laboratories. Delegates are encouraged to bring questions, separation problems and other topics of interest to discuss during the course as a group or one-to-one with the instructor.
 
Available as an in-house or off-site course.