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Introduction

Exploring the Power of Eluent pH in LC Method Development

Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in analyte retention from eluent pH can be helpful for screening systems and method development platforms where observing the number of components in samples is important before method optimisation is attempted. The chromatographic selectivity 'space' for various eluent and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included.

• Exploring chromatographic selectivity
• Stationary phase design
• Acidic and basic analyte ionisation theory
• Choosing appropriate stationary phases
• HPLC and UHPLC
• Practical hints and tips

Deadlines

• Feb 09 2015
  Early bird registration deadline

  

Useful links

Downloads

• Hichrom Registration form

Speakers

• Alan McKeown Advanced Chromatography Technologies Ltd, United Kingdom

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Venue

Birmingham University

Malvern Room, Horton Grange, Birmingham University, 53 Edgbaston Park Road, Birmingham, B15 2RS, United Kingdom

Organised by

Hichrom Ltd

Hichrom Ltd

Contact information

Jenny Sumner
Hichrom Ltd
1-3 The Markham Centre
Station Road
Theale
Reading
RG7 4PE
01189303660
Contact us by email

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